New assay helps determine lymphoma subtypes simply, quickly, and inexpensively

16 abril 2015

With the advent of targeted lymphoma therapies on the horizon, it becomes increasingly important to differentiate the two major subtypes of diffuse large B-cell lymphoma (DLBCL), which is the most common non-Hodgkin lymphoma. These are germinal center B-cell-like (GCB) and activated B-cell-like (ABC), which differ in management and outcomes. A report describes use of the reverse transcriptase multiplex ligation-dependent probe amplification (RT-MLPA) assay for differentiating DLBCL subtypes. RT-MLPA is as accurate as the current gold standard technology and offers advantages such as simplicity, flexibility, short turnaround time, low cost, and efficiency.